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e coli ec1000 (Addgene inc)

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Addgene inc e coli ec1000
E Coli Ec1000, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ec1000/EC1000+(Bacterial+strain+%2371852)/10__1093_slash_femsml_slash_uqag009-71-24-27
Average 93 stars, based on 6 article reviews

e coli ec1000 - by Bioz Stars, 2026-09

93/100 stars

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Plasmid Preparation:

Article Title: Vaginal Lactobacillus fatty acid response mechanisms reveal a metabolite-targeted strategy for bacterial vaginosis treatment.
Article Snippet: .. EC1000 and pORI28 were gifts from Todd Klaenhammer and Jan Kok (respectively, Addgene plasmid #71852 and Addgene plasmid #71595; http://n2t.net/ addgene:71595; RRID:Addgene_71595). pTRK892 and pTRK669 were gifts from Rodolphe Barrangou and Todd Klaenhammer (respectively, Addgene plasmid #71803; http://n2t.net/addgene:71803; RRID:Addgene_71803 and Addgene plasmid #71313; http://n2t.net/addgene:71313; and RRID:Addgene_71313). .. Bulk RNA sequencing samples were processed and data were generated by the Infectious Disease and Microbiome Program’s Microbial Omics Core at the Broad Institute ofMIT andHarvard.

Cloning:

Article Title: Vaginal Lactobacillus fatty acid response mechanisms reveal a metabolite-targeted strategy for bacterial vaginosis treatment.
Article Snippet: .. EC1000, E. coli cloning host for pORI28 plasmids, RepA+ Addgene 71852 MC1061 Competent E. coli, E. coli cloning host for pTRK892 plasmids Molecular Cloning Laboratories MC1061 ATCC 33323, L. gasseri type strain ATCC 33323 ATCC 4356, L. acidophilus type strain ATCC 4356 ATCC 33323 with a clean gene deletion of LGAS_1245 This study Wild-type (WT) ATCC 33323 with an in-frame gene deletion of LGAS_1351 This study DohyA9 ATCC 33323 with an in-frame gene deletion of LGAS_1630 This study DfarE ATCC 33323 with an in-frame gene deletion of LGAS_1351, complemented with pMZ12 This study DohyA9/pohyA9 ATCC 33323 with an in-frame gene deletion of LGAS_1630, complemented with pMZ13 This study DfarE/pfarE Laboratory S. aureus strain that can accept DNA from Escherichia coli. ..

Article Title: Vaginal Lactobacillus fatty acid response mechanisms reveal a metabolite-targeted strategy for bacterial vaginosis treatment.
Article Snippet: .. Escherichia coli cloning strains, EC1000 (Addgene #71852) and MC1061 (Molecular Cloning Laboratories #MC1061), S. aureus, and S. aureus derivative mutant strains were cultured under aerobic conditions at 37 C with shaking for liquid cultures. .. Escherichia coli cloning strains, EC1000 (Addgene #71852) and MC1061 (Molecular Cloning Laboratories #MC1061), S. aureus, and S. aureus derivative mutant strains were cultured under aerobic conditions at 37 C with shaking for liquid cultures.

Article Title: Vaginal Lactobacillus fatty acid response mechanisms reveal a novel strategy for bacterial vaginosis treatment
Article Snippet: .. Escherichia coli cloning strains, EC1000 (Addgene #71852) and MC1061 (Molecular Cloning Laboratories #MC1061), Staphylococcus aureus , and S. aureus derivative mutant strains were cultured under aerobic conditions at 37°C with shaking for liquid cultures. ..

Article Title: Vaginal Lactobacillus fatty acid response mechanisms reveal a metabolite-targeted strategy for bacterial vaginosis treatment
Article Snippet: .. EC1000, E. coli cloning host for pORI28 plasmids, RepA+ , Addgene , 71852. .. MC1061 Competent E. coli, E. coli cloning host for pTRK892 plasmids , Molecular Cloning Laboratories , MC1061.

Article Title: Vaginal Lactobacillus fatty acid response mechanisms reveal a metabolite-targeted strategy for bacterial vaginosis treatment
Article Snippet: .. Escherichia coli cloning strains, EC1000 (Addgene #71852) and MC1061 (Molecular Cloning Laboratories #MC1061), S. aureus , and S. aureus derivative mutant strains were cultured under aerobic conditions at 37°C with shaking for liquid cultures. ..

Molecular Cloning:

Article Title: Vaginal Lactobacillus fatty acid response mechanisms reveal a metabolite-targeted strategy for bacterial vaginosis treatment.
Article Snippet: .. EC1000, E. coli cloning host for pORI28 plasmids, RepA+ Addgene 71852 MC1061 Competent E. coli, E. coli cloning host for pTRK892 plasmids Molecular Cloning Laboratories MC1061 ATCC 33323, L. gasseri type strain ATCC 33323 ATCC 4356, L. acidophilus type strain ATCC 4356 ATCC 33323 with a clean gene deletion of LGAS_1245 This study Wild-type (WT) ATCC 33323 with an in-frame gene deletion of LGAS_1351 This study DohyA9 ATCC 33323 with an in-frame gene deletion of LGAS_1630 This study DfarE ATCC 33323 with an in-frame gene deletion of LGAS_1351, complemented with pMZ12 This study DohyA9/pohyA9 ATCC 33323 with an in-frame gene deletion of LGAS_1630, complemented with pMZ13 This study DfarE/pfarE Laboratory S. aureus strain that can accept DNA from Escherichia coli. ..

Article Title: Vaginal Lactobacillus fatty acid response mechanisms reveal a metabolite-targeted strategy for bacterial vaginosis treatment.
Article Snippet: .. Escherichia coli cloning strains, EC1000 (Addgene #71852) and MC1061 (Molecular Cloning Laboratories #MC1061), S. aureus, and S. aureus derivative mutant strains were cultured under aerobic conditions at 37 C with shaking for liquid cultures. .. Escherichia coli cloning strains, EC1000 (Addgene #71852) and MC1061 (Molecular Cloning Laboratories #MC1061), S. aureus, and S. aureus derivative mutant strains were cultured under aerobic conditions at 37 C with shaking for liquid cultures.

Article Title: Vaginal Lactobacillus fatty acid response mechanisms reveal a metabolite-targeted strategy for bacterial vaginosis treatment
Article Snippet: .. Escherichia coli cloning strains, EC1000 (Addgene #71852) and MC1061 (Molecular Cloning Laboratories #MC1061), S. aureus , and S. aureus derivative mutant strains were cultured under aerobic conditions at 37°C with shaking for liquid cultures. ..

Mutagenesis:

Article Title: Vaginal Lactobacillus fatty acid response mechanisms reveal a metabolite-targeted strategy for bacterial vaginosis treatment.
Article Snippet: .. Escherichia coli cloning strains, EC1000 (Addgene #71852) and MC1061 (Molecular Cloning Laboratories #MC1061), S. aureus, and S. aureus derivative mutant strains were cultured under aerobic conditions at 37 C with shaking for liquid cultures. .. Escherichia coli cloning strains, EC1000 (Addgene #71852) and MC1061 (Molecular Cloning Laboratories #MC1061), S. aureus, and S. aureus derivative mutant strains were cultured under aerobic conditions at 37 C with shaking for liquid cultures.

Article Title: Vaginal Lactobacillus fatty acid response mechanisms reveal a metabolite-targeted strategy for bacterial vaginosis treatment
Article Snippet: .. Escherichia coli cloning strains, EC1000 (Addgene #71852) and MC1061 (Molecular Cloning Laboratories #MC1061), S. aureus , and S. aureus derivative mutant strains were cultured under aerobic conditions at 37°C with shaking for liquid cultures. ..

Cell Culture:

Article Title: Vaginal Lactobacillus fatty acid response mechanisms reveal a metabolite-targeted strategy for bacterial vaginosis treatment.
Article Snippet: .. Escherichia coli cloning strains, EC1000 (Addgene #71852) and MC1061 (Molecular Cloning Laboratories #MC1061), S. aureus, and S. aureus derivative mutant strains were cultured under aerobic conditions at 37 C with shaking for liquid cultures. .. Escherichia coli cloning strains, EC1000 (Addgene #71852) and MC1061 (Molecular Cloning Laboratories #MC1061), S. aureus, and S. aureus derivative mutant strains were cultured under aerobic conditions at 37 C with shaking for liquid cultures.

Article Title: Vaginal Lactobacillus fatty acid response mechanisms reveal a metabolite-targeted strategy for bacterial vaginosis treatment
Article Snippet: .. Escherichia coli cloning strains, EC1000 (Addgene #71852) and MC1061 (Molecular Cloning Laboratories #MC1061), S. aureus , and S. aureus derivative mutant strains were cultured under aerobic conditions at 37°C with shaking for liquid cultures. ..

Image Search Results

MinD oscillation in cells of different lengths. (A) Time-lapse micrographs illustrating the pole-to-pole oscillation of sfGFP-MinD in cells of different lengths. The exponentially growing E. coli strain FW1541 was imaged at 12-s intervals for 10 min. Scale bar: 2 μm. (B) Standing wave features are demonstrated at 10% and 25% of the cell length, which was equivalent to the positions at 90% and 75%, respectively, along the medial axes of cells. (C) Kymographs demonstrating changes in the intensity profiles with length. (D) The standard deviation (SD) of the oscillation period is reduced in longer cells. (E and G) The correlation plots between the oscillation period and cell length when cultured with 0.416% (E) and 0% glucose (G). The oscillation periods range from 37 to 66 s for 0.416% glucose and from 41 to 99 s for 0% glucose. (F) Correlation between the oscillation velocity and the cell cycle duration. In A–C, MinD oscillations in three representative cells with varying lengths are presented. In D–G, analyses based on pooled data from multiple image fields are presented to demonstrate the oscillatory dynamics across cells of different lengths. Correlations were calculated using the nonparametric Spearman correlation method with 95% confidence intervals. The red dashed lines represent the lines fitted by simple linear regression. R 2 , goodness of fit; r, Spearman coefficient; P: two-tailed probability; n , population size. D–F, n = 130; G, n = 101.

Journal: The Journal of Cell Biology

Article Title: Growth-dependent concentration gradient of the oscillating Min system in Escherichia coli

doi: 10.1083/jcb.202406107

Figure Lengend Snippet: MinD oscillation in cells of different lengths. (A) Time-lapse micrographs illustrating the pole-to-pole oscillation of sfGFP-MinD in cells of different lengths. The exponentially growing E. coli strain FW1541 was imaged at 12-s intervals for 10 min. Scale bar: 2 μm. (B) Standing wave features are demonstrated at 10% and 25% of the cell length, which was equivalent to the positions at 90% and 75%, respectively, along the medial axes of cells. (C) Kymographs demonstrating changes in the intensity profiles with length. (D) The standard deviation (SD) of the oscillation period is reduced in longer cells. (E and G) The correlation plots between the oscillation period and cell length when cultured with 0.416% (E) and 0% glucose (G). The oscillation periods range from 37 to 66 s for 0.416% glucose and from 41 to 99 s for 0% glucose. (F) Correlation between the oscillation velocity and the cell cycle duration. In A–C, MinD oscillations in three representative cells with varying lengths are presented. In D–G, analyses based on pooled data from multiple image fields are presented to demonstrate the oscillatory dynamics across cells of different lengths. Correlations were calculated using the nonparametric Spearman correlation method with 95% confidence intervals. The red dashed lines represent the lines fitted by simple linear regression. R 2 , goodness of fit; r, Spearman coefficient; P: two-tailed probability; n , population size. D–F, n = 130; G, n = 101.

Article Snippet: The E. coli strains MC1000 ( araD139 Δ(araABC-leu)7679 galU galK Δ(lac)X74 rpsL thi ; RRID: Addgene_71852) ( ) and DH5α ( ΔlacZ ΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17(rK-mK+) supE44 thi-1 gyrA96 relA1 ; RRDI: SCR_006368) ( ) were used for general cloning purposes.

Techniques: Standard Deviation, Cell Culture, Two Tailed Test

Characteristic features of E. coli strains FW1541 and W3110 cultured in M9 medium supplemented with 0.416% glucose at 30°C. (A) Comparison of cell length, width, and aspect ratio. Data are presented as median values with interquartile ranges. Pooled data from several image fields were used for the analyses. Statistical comparisons were made using unpaired nonparametric Mann–Whitney tests. The median values are labeled and shown as the dashed lines, while the solid lines indicate the interquartile ranges. The P values of each pairwise comparison are labeled on top. W3110, n = 943; FW1541, n = 941. (B) Cell area (left) and aspect ratio (right), respectively, plotted against length and width. Correlations were calculated using the nonparametric Spearman correlation method with 95% confidence intervals. The same image dataset as in was used for the analysis. r, Spearman coefficient; P: two-tailed probability; n , population size. (C) A positive correlation between cell length and time was observed based on experimental data collected at 15-min intervals. The same image dataset as in , covering a complete cell cycle, was used for the analysis. Simple linear regression was applied to determine the length increase over time. The red line represents the best-fit regression line, while the blue dashed lines represent the errors. (D) A correlation plot of the period versus time was generated based on data from and . The Spearman nonparametric correlation method was used to calculate the correlation with 95% confidence intervals. The red dashed line represents the best-fit regression line. (B−D) R 2 , goodness of fit; r, Spearman coefficient; P: two-tailed; probability. n , population size. (E) Illustration of the cell volume calculation of FW1541.

Journal: The Journal of Cell Biology

Article Title: Growth-dependent concentration gradient of the oscillating Min system in Escherichia coli

doi: 10.1083/jcb.202406107

Figure Lengend Snippet: Characteristic features of E. coli strains FW1541 and W3110 cultured in M9 medium supplemented with 0.416% glucose at 30°C. (A) Comparison of cell length, width, and aspect ratio. Data are presented as median values with interquartile ranges. Pooled data from several image fields were used for the analyses. Statistical comparisons were made using unpaired nonparametric Mann–Whitney tests. The median values are labeled and shown as the dashed lines, while the solid lines indicate the interquartile ranges. The P values of each pairwise comparison are labeled on top. W3110, n = 943; FW1541, n = 941. (B) Cell area (left) and aspect ratio (right), respectively, plotted against length and width. Correlations were calculated using the nonparametric Spearman correlation method with 95% confidence intervals. The same image dataset as in was used for the analysis. r, Spearman coefficient; P: two-tailed probability; n , population size. (C) A positive correlation between cell length and time was observed based on experimental data collected at 15-min intervals. The same image dataset as in , covering a complete cell cycle, was used for the analysis. Simple linear regression was applied to determine the length increase over time. The red line represents the best-fit regression line, while the blue dashed lines represent the errors. (D) A correlation plot of the period versus time was generated based on data from and . The Spearman nonparametric correlation method was used to calculate the correlation with 95% confidence intervals. The red dashed line represents the best-fit regression line. (B−D) R 2 , goodness of fit; r, Spearman coefficient; P: two-tailed; probability. n , population size. (E) Illustration of the cell volume calculation of FW1541.

Techniques: Cell Culture, Comparison, MANN-WHITNEY, Labeling, Two Tailed Test, Generated

Examination of FtsA localization, which marks the division site, in relation to cell length and MinD oscillation period in E. coli strain FW1541. (A) Representative images demonstrating simultaneous observation of FtsA-mScarlet-I, sfGFP-MinD, and cell morphology. (B and C) The ring-like structure of FtsA-mScarlet-I had a relative position of 47.5 ± 2.1% from either pole (B), regardless of the cell length and the period of the MinD oscillations (C). The cell center is set at position 0.5 for normalization of the FtsA position. Pooled data from several image fields were used for the analysis. The Spearman nonparametric correlation method was used to calculate the correlation with 95% confidence intervals. The red dashed line represents the best-fit linear regression line. n , population size; R 2 , goodness of fit; r, Spearman coefficient; P: two-tailed probability.

Journal: The Journal of Cell Biology

Article Title: Growth-dependent concentration gradient of the oscillating Min system in Escherichia coli

doi: 10.1083/jcb.202406107

Figure Lengend Snippet: Examination of FtsA localization, which marks the division site, in relation to cell length and MinD oscillation period in E. coli strain FW1541. (A) Representative images demonstrating simultaneous observation of FtsA-mScarlet-I, sfGFP-MinD, and cell morphology. (B and C) The ring-like structure of FtsA-mScarlet-I had a relative position of 47.5 ± 2.1% from either pole (B), regardless of the cell length and the period of the MinD oscillations (C). The cell center is set at position 0.5 for normalization of the FtsA position. Pooled data from several image fields were used for the analysis. The Spearman nonparametric correlation method was used to calculate the correlation with 95% confidence intervals. The red dashed line represents the best-fit linear regression line. n , population size; R 2 , goodness of fit; r, Spearman coefficient; P: two-tailed probability.

Techniques: Two Tailed Test

Journal: iScience

Article Title: Lasso peptides sviceucin and siamycin I exhibit anti-virulence activity and restore vancomycin effectiveness in vancomycin-resistant pathogens

doi: 10.1016/j.isci.2025.111922

Figure Lengend Snippet:

Article Snippet: Escherichia coli EC1000 , Leenhouts et al. ; Addgene , Cat# 71852.

Techniques: Virus, Recombinant, Staining, Plasmid Preparation, Lysis, Reverse Transcription, Bicinchoninic Acid Protein Assay, Software, Chromatography, Real-time Polymerase Chain Reaction

Journal: Cell

Article Title: Vaginal Lactobacillus fatty acid response mechanisms reveal a metabolite-targeted strategy for bacterial vaginosis treatment

doi: 10.1016/j.cell.2024.07.029

Figure Lengend Snippet:

Article Snippet: EC1000, E. coli cloning host for pORI28 plasmids, RepA+ , Addgene , 71852.

Techniques: Virus, Cloning, Molecular Cloning, Mutagenesis, Expressing, Plasmid Preparation, Recombinant, Electron Microscopy, Purification, Saline, Reverse Transcription, Clarification Assay, Viscosity, Gel Extraction, Transformation Assay, Electroporation, RNA Sequencing, Sequencing, Software, Transmission Assay, Microscopy

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e coli ec1000 (Addgene inc)

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